il 1β 172 (Cell Signaling Technology Inc)
Structured Review
Il 1β 172, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 234 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/il+1%CE%B2/IL-1beta+Rabbit+mAb/pm42815593-78-50-53
Average 96 stars, based on 234 article reviews
Images
Related Articles
Incubation:Article Title: Erchen decoction and its active flavonoids hesperidin and quercetin alleviate high-fat diet-induced neuroinflammation by targeting HK2-mediated microglial glycolysis. Article Snippet: Ethnopharmacological relevance: Erchen decoction (ECD), a classic Traditional Chinese Medicine formula recorded in the Taiping Huimin Heji Ju Fang, is historically recognized as the fundamental prescription for drying dampness and resolving phlegm.. While ECD is widely used in modern clinical practice for metabolic disorders, the pharmacological mechanisms linking its traditional indications to the regulation of obesity-associated neuroinflammation remain elusive.. Aim of the study: This study aimed to systematically characterize the bioactive phytochemicals of ECD and elucidate its neuroprotective mechanism against high-fat diet (HFD)-induced neuroinflammation, with a specific focus on reprogramming microglial immunometabolism via the hexokinase 2 (HK2) checkpoint. Article Title: Cannabidiol hinders lipopolysaccharide-induced neutrophils migration to the lungs through suppressing nuclear factor kappa-B signal and expression of interleukin-1 beta in macrophages. Article Snippet: Background: Acute lung injury and its more severe form, acute respiratory distress syndrome, are life-threatening diseases characterized by uncontrolled pulmonary inflammation, impaired gas exchange, and high mortality rates.. Effective therapeutic agents remain limited.. As a non-addictive component derived from hemp seed, the anti-inflammatory activity of cannabidiol (CBD) has been suggested by multiple pathological models. Article Title: Polyethylene terephthalate microplastics impair erectile function through macrophage mediated cGAS-STING ferroptosis Article Snippet: Protein concentration was measured using a BCA assay kit (Beyotime, China). .. Equal amounts of protein (30 μg) were separated on 10% SDS-PAGE gels at 120 V for 2 h and transferred onto NC membranes at 400 mA for 1 h. Membranes were blocked with 5% non-fat milk at room temperature for 2 h and incubated overnight at 4°C with primary antibodies against TGF-β (Abcam, ab92486, 1:1000), α-SMA (Abcam, ab7817, 1:5000), eNOS (Cell Signaling Technology, CST #9572, 1:1000), p-eNOS (CST #9571, 1:1000), β-tubulin (Proteintech, 10068-1-AP, 1:5000), Article Title: Matrine improves bile acid metabolism and reduces inflammatory and oxidative stress in colitis via the JAK2 pathway. Article Snippet: .. Membrane was blocked within Odyssey blocking buffer (LI-COR Bioscience, Lincoln, USA) for 1 h at room temperature (RT), and then incubated with primary antibodies against p-JAK2 (AP0917, Abclonal, Woburn, USA), JAK2 (AF6022, Affinitiy Bioscience, Changzhou, China), MRP3 (bs-0656R, Bioss, Beijing, China), MRP4 (DF6921, Affinity Bioscience), Article Title: Study on the Effects of Transcutaneous Auricular Acupuncture Stimulation of the Vagus Nerve on Post-Traumatic Cerebral Oedema Article Snippet: Proteins were subsequently transferred to Polyvinylidene Fluoride (PVDF) membranes (Beyotime, Shanghai, China) using a semi-dry transfer method (PVDF membranes were activated with methanol prior to transfer; both gel and membrane were equilibrated in ice-cold transfer buffer). .. It was then incubated sequentially with specific primary antibodies (glyceraldehyde-3phosphate dehydrogenase (GAPDH, 1:8000, 60004-1-Ig, Proteintech Group, Inc., Rosemont, IL, USA); HMGB1 (1:1000, ab18256, abcam, Cambridge, UK); Article Title: Matrine improves bile acid metabolism and reduces inflammatory and oxidative stress in colitis via the JAK2 pathway Article Snippet: .. Membrane was blocked within Odyssey blocking buffer (LI-COR Bioscience, Lincoln, USA) for 1 h at room temperature (RT), and then incubated with primary antibodies against p-JAK2 (AP0917, Abclonal, Woburn, USA), JAK2 (AF6022, Affinitiy Bioscience, Changzhou, China), MRP3 (bs-0656R, Bioss, Beijing, China), MRP4 (DF6921, Affinity Bioscience), other:Article Title: Adipose extracellular vesicles carrying miR-210-3p drive macrophage inflammation and nicotine-induced atherosclerosis Article Snippet: SDS Page:Article Title: Polyethylene terephthalate microplastics impair erectile function through macrophage mediated cGAS-STING ferroptosis Article Snippet: Protein concentration was measured using a BCA assay kit (Beyotime, China). .. Equal amounts of protein (30 μg) were separated on 10% SDS-PAGE gels at 120 V for 2 h and transferred onto NC membranes at 400 mA for 1 h. Membranes were blocked with 5% non-fat milk at room temperature for 2 h and incubated overnight at 4°C with primary antibodies against TGF-β (Abcam, ab92486, 1:1000), α-SMA (Abcam, ab7817, 1:5000), eNOS (Cell Signaling Technology, CST #9572, 1:1000), p-eNOS (CST #9571, 1:1000), β-tubulin (Proteintech, 10068-1-AP, 1:5000), Pyrolysis Gas Chromatography:Article Title: Polyethylene terephthalate microplastics impair erectile function through macrophage mediated cGAS-STING ferroptosis Article Snippet: Protein concentration was measured using a BCA assay kit (Beyotime, China). .. Equal amounts of protein (30 μg) were separated on 10% SDS-PAGE gels at 120 V for 2 h and transferred onto NC membranes at 400 mA for 1 h. Membranes were blocked with 5% non-fat milk at room temperature for 2 h and incubated overnight at 4°C with primary antibodies against TGF-β (Abcam, ab92486, 1:1000), α-SMA (Abcam, ab7817, 1:5000), eNOS (Cell Signaling Technology, CST #9572, 1:1000), p-eNOS (CST #9571, 1:1000), β-tubulin (Proteintech, 10068-1-AP, 1:5000), Membrane:Article Title: Matrine improves bile acid metabolism and reduces inflammatory and oxidative stress in colitis via the JAK2 pathway. Article Snippet: .. Membrane was blocked within Odyssey blocking buffer (LI-COR Bioscience, Lincoln, USA) for 1 h at room temperature (RT), and then incubated with primary antibodies against p-JAK2 (AP0917, Abclonal, Woburn, USA), JAK2 (AF6022, Affinitiy Bioscience, Changzhou, China), MRP3 (bs-0656R, Bioss, Beijing, China), MRP4 (DF6921, Affinity Bioscience), Article Title: Matrine improves bile acid metabolism and reduces inflammatory and oxidative stress in colitis via the JAK2 pathway Article Snippet: .. Membrane was blocked within Odyssey blocking buffer (LI-COR Bioscience, Lincoln, USA) for 1 h at room temperature (RT), and then incubated with primary antibodies against p-JAK2 (AP0917, Abclonal, Woburn, USA), JAK2 (AF6022, Affinitiy Bioscience, Changzhou, China), MRP3 (bs-0656R, Bioss, Beijing, China), MRP4 (DF6921, Affinity Bioscience), Blocking Assay:Article Title: Matrine improves bile acid metabolism and reduces inflammatory and oxidative stress in colitis via the JAK2 pathway. Article Snippet: .. Membrane was blocked within Odyssey blocking buffer (LI-COR Bioscience, Lincoln, USA) for 1 h at room temperature (RT), and then incubated with primary antibodies against p-JAK2 (AP0917, Abclonal, Woburn, USA), JAK2 (AF6022, Affinitiy Bioscience, Changzhou, China), MRP3 (bs-0656R, Bioss, Beijing, China), MRP4 (DF6921, Affinity Bioscience), Article Title: Huazhuo Decoction Contributes to Immune Homeostasis and Attenuates Neuronal Inflammation and Demyelination in a Mouse Model of Multiple Sclerosis Article Snippet: Following separation on 10% SDS-PAGE gels, proteins were electrophoretically transferred to PVDF membranes (IPVH0010, Millipore, US). .. Membranes were blocked for 30 min at room temperature using a rapid blocking buffer (PS108P, Epizyme, China) and then incubated overnight at 4°C with anti-NLRP3 (ab270449, Abcam, USA), NF-κB (8242S, CST, USA), Article Title: Matrine improves bile acid metabolism and reduces inflammatory and oxidative stress in colitis via the JAK2 pathway Article Snippet: .. Membrane was blocked within Odyssey blocking buffer (LI-COR Bioscience, Lincoln, USA) for 1 h at room temperature (RT), and then incubated with primary antibodies against p-JAK2 (AP0917, Abclonal, Woburn, USA), JAK2 (AF6022, Affinitiy Bioscience, Changzhou, China), MRP3 (bs-0656R, Bioss, Beijing, China), MRP4 (DF6921, Affinity Bioscience), Control:Article Title: Matrine improves bile acid metabolism and reduces inflammatory and oxidative stress in colitis via the JAK2 pathway. Article Snippet: .. Membrane was blocked within Odyssey blocking buffer (LI-COR Bioscience, Lincoln, USA) for 1 h at room temperature (RT), and then incubated with primary antibodies against p-JAK2 (AP0917, Abclonal, Woburn, USA), JAK2 (AF6022, Affinitiy Bioscience, Changzhou, China), MRP3 (bs-0656R, Bioss, Beijing, China), MRP4 (DF6921, Affinity Bioscience), Article Title: Huazhuo Decoction Contributes to Immune Homeostasis and Attenuates Neuronal Inflammation and Demyelination in a Mouse Model of Multiple Sclerosis Article Snippet: Following separation on 10% SDS-PAGE gels, proteins were electrophoretically transferred to PVDF membranes (IPVH0010, Millipore, US). .. Membranes were blocked for 30 min at room temperature using a rapid blocking buffer (PS108P, Epizyme, China) and then incubated overnight at 4°C with anti-NLRP3 (ab270449, Abcam, USA), NF-κB (8242S, CST, USA), Article Title: Matrine improves bile acid metabolism and reduces inflammatory and oxidative stress in colitis via the JAK2 pathway Article Snippet: .. Membrane was blocked within Odyssey blocking buffer (LI-COR Bioscience, Lincoln, USA) for 1 h at room temperature (RT), and then incubated with primary antibodies against p-JAK2 (AP0917, Abclonal, Woburn, USA), JAK2 (AF6022, Affinitiy Bioscience, Changzhou, China), MRP3 (bs-0656R, Bioss, Beijing, China), MRP4 (DF6921, Affinity Bioscience), |
